April 25, 2026

Honolulu Zoo’s Sumatran tiger undergoes artificial insemination

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HONOLULU — On Saturday, 6-year-old Anala, Honolulu Zoo’s resident Sumatran tiger, underwent a “novel artificial insemination procedure” with the assistance of a team of reproductive specialists from Omaha’s Henry Doorly Zoo, according to a news release.


What You Need To Know

    • The novel procedure, laparoscopic oviductal artificial insemination, has only been performed in a handful of tigers, according to the Honolulu Zoo
    • AI has led to successful births in other species, such as domestic cats, wolves, rhinos, oryx, clouded leopards and the Tsushima leopard cat
  • If Anala doesn’t become pregnant through the AI procedure, the Honolulu Zoo will welcome a male tiger as a potential mate in 2024

Anala’s keepers helped prepare her for the procedure by collecting daily fecal samples and observing behavior changes to track her hormone cycling to determine her ovulation date. They also trained her to accept voluntary injections and oral medication.

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